Inhibiting homologous recombination for cancer therapy
نویسندگان
چکیده
منابع مشابه
Designing E1 Deleted Adenoviral Vector by Homologous Recombination
Adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. In spite of their usefulness, the construction of adenoviral vectors (AdV) is a cumbersome and lengthy process that is not readily amenable to the generat...
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A report in PNAS suggests that mild hyperthermia can inhibit the process of homologous recombination (HR) and degrade the BRCA2 protein (1). Cellular hyperthermia, up to temperatures of 41 °C to 43 °C, has long been known to be a potent radiosensitizer of proliferating cells, providing the opportunity to enhance the tumoricidal effects of therapeutical radiation, because most nonmalignant cells...
متن کاملHomologous recombination
DNA damage, in the form of DNA double-strand breaks, poses a considerable threat to genomic integrity and cell survival. If left unrepaired, a single double-strand break is sufficient to cause cell death and, if repaired inappropriately, a double-strand break may give rise to a potentially oncogenic translocation. Double-strand breaks in genomic DNA may arise accidentally in a number of ways, i...
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Homologous recombination can be divided into three key steps: strand exchange, branch migration and resolution. The identification of a protein complex that catalyses branch migration and Holliday junction resolution argues that the mechanism of homologous recombination is conserved from bacteria to man.
متن کاملan improved homologous recombination method for rapid cloning of the antibody heavy chain gene and its comparison with the homologous recombination and traditional cloning methods
background: the homologous recombination (hr) is one of the conventional cloning methods for the production of recombinant dna. it is a quick method for in vivo dna cloning without using the high cost restriction enzymes. a few modifications in the cloning procedure can increase the efficiency of this method. m aterials and methods: in this study, effect of heating on the rate of the igg1 heavy...
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ژورنال
عنوان ژورنال: Cancer Biology & Therapy
سال: 2012
ISSN: 1538-4047,1555-8576
DOI: 10.4161/cbt.13.2.18872